DeNovoMAGIC™2.0, which incorporates novel algorithms, significantly improves the assembly result with much shorter turnaround time and lower cost. For the assembly of Tausch's goatgrass (Aegilops tauschii), by using DeNovoMAGIC™2.0, NRGene has improved scaffold N50 to 11.4Mb, which is 200 times better than 57.60Kb that was published in the Nature journal. The assembly process that utilizes cloud computing technology only takes 5 days, compared to 2-8 months run time used by previous technologies.
Below is a list of selected projects, from which you will see the first-ever reference genomes for previously unmapped plants and animals.
Maize(W22) assembly result | ||||
---|---|---|---|---|
Parameter | DeNovoMAGIC™ 2.0 | DeNovoMAGIC™ 2.0+10X | ||
Scaffold N50 (L50) |
9.4 Mbp(68) | 35.5 Mbp(18) | ||
Scaffold N90 (L90) |
1.95 Mbp(256) | 11 Mbp(58) | ||
Total Assembly Size | 1.18 Gbp | 2.13 Gbp | ||
GAPs(=n) | 1.88% | 1.90% | ||
Chimeric scaffolds | 30 (0.1% miss assembly) | 0 |
DeNovoMAGIC™ 2.0 (without 10X Genomics)
Parameter | Diploid Wheat (Aegilops tauschii) |
Hexaploid Wheat (Chinese Spring) |
Tomato |
---|---|---|---|
Fold Coverage of Short Reads (PE&MP) |
200X | 180X | 190X |
Run Time - Days | 5 | 14 | 2 |
Contig N50 | 69 kbp | 52 kbp | 65 kbp |
Scaffold N50 (L50) |
11.4 Mbp(106) | 7.06 Mbp(566) | 10.4 Mbp(21) |
Scaffold N90 (L90) |
27 Mbp(405) | 1.26 Mbp(2363) | 1.87 Mbp(92) |
Total Assembly Size | 4.09 Gbp | 14.53 Gbp | 0.82 Gbp |
GAPs(=n) | 1.39% | 1.80% | 1.62% |