Combination of 10x Genomics data produces high quality genome

DeNovoMAGIC™2.0, which incorporates novel algorithms, significantly improves the assembly result with much shorter turnaround time and lower cost. For the assembly of Tausch's goatgrass (Aegilops tauschii), by using DeNovoMAGIC™2.0, NRGene has improved scaffold N50 to 11.4Mb, which is 200 times better than 57.60Kb that was published in the Nature journal. The assembly process that utilizes cloud computing technology only takes 5 days, compared to 2-8 months run time used by previous technologies.

Below is a list of selected projects, from which you will see the first-ever reference genomes for previously unmapped plants and animals.

Maize(W22) assembly result
Parameter DeNovoMAGIC™ 2.0 DeNovoMAGIC™ 2.0+10X
Scaffold N50
(L50)
9.4 Mbp(68) 35.5 Mbp(18)
Scaffold N90
(L90)
1.95 Mbp(256) 11 Mbp(58)
Total Assembly Size 1.18 Gbp 2.13 Gbp
GAPs(=n) 1.88% 1.90%
Chimeric scaffolds 30 (0.1% miss assembly) 0

DeNovoMAGIC™ 2.0 (without 10X Genomics)

Parameter Diploid Wheat
(Aegilops tauschii)
Hexaploid Wheat
(Chinese Spring)
Tomato
Fold Coverage
of Short Reads
(PE&MP)
200X 180X 190X
Run Time - Days 5 14 2
Contig N50 69 kbp 52 kbp 65 kbp
Scaffold N50
(L50)
11.4 Mbp(106) 7.06 Mbp(566) 10.4 Mbp(21)
Scaffold N90
(L90)
27 Mbp(405) 1.26 Mbp(2363) 1.87 Mbp(92)
Total Assembly Size 4.09 Gbp 14.53 Gbp 0.82 Gbp
GAPs(=n) 1.39% 1.80% 1.62%