GENOSYS & NRGene provide perfect genomes

NRGene

NRGene, a leading genomic big data company, develops advanced computational tools and cutting-edge algorithmic models to facilitate optimal trait discovery for seed companies, animal breeders, and academia. NRGene’s DeNovoMAGIC TM assembles whole genomes from short reads, generating long and phased scaffolds, delivering accurate assembly results even for the most complex genomes. It is ideal for high-quality genome mapping and productive breeding.

Genosys

Genosys is a leading genomics solution provider focusing on helping our customers to interpret reliable results from NGS data. We have built ground-breaking, efficient and user-friendly bioinformatics analytical tools and have vast experience in the interpretation of multi-omics data. Genosys is an authorized distributor of NRGene in Asia-Pacific. With NRGene’s DeNovoMAGIC and Genosys providing subsequent bioinformatics services, the genome-wide study of various species will become much more cost effective and efficient.

Example Assembly Results (>80 Genomes Delivered)

Parameter Diploid Wheat
(Aegilops tauschii)		 Tetraploid Wheat
(Wild Emmer)		 Hexaploid Wheat
(Chinese Spring)		 Maize Tomato Rainbow Trout Wild Soybean
Fold Coverage
of Short Reads
(PE&MP)		 200X 180X 180X 180 190X 230X 230X
Run Time - Days 5 10 14 2 2 2 1
Contig N50 69 kbp 57 kbp 52 kbp 73 kbp 65 kbp 24 kbp 24 kbp
Scaffold N50
(L50)		 11.4 Mbp
(106)		 7.0 Mbp
414)		 7.06 Mbp
(566)		 9.4 Mbp
(68)		 10.4 Mbp
(21)		 2.0 Mbp
(236)		 4.68 Mbp
(57)
Scaffold N90
(L90)		 27 Mbp
(405)		 1.15 Mbp
(1827)		 1.26 Mbp
(2363)		 1.95 Mbp
(256)		 1.87 Mbp
(92)		 0.06 Kbp
(3479)		 0.72 Mbp
(260)
Total Assembly Size 4.09 Gbp 10.50 Gbp 14.53 Gbp 2.18 Gbp 0.82 Gbp 2.31 Gbp 0.997 Gbp
Unfilled Gaps(=n) 1.39% 1.63% 1.80% 1.88% 1.62% 14.40% 3.5%

Comparison of Assembly Results Between Publications and NRGene

Parameter Assembly Results from Publications Assembly Results from NRGene
Diploid Wheat
(Aegilops tauschii)		 Rainbow Trout
(Oncorhynchus mykiss)		 Diploid Wheat
(Aegilops tauschii)		 Rainbow Trout
(Oncorhynchus mykiss)
Fold Coverage
of Short Reads
(PE&MP)		 90X 70X 200X 230
Contig N50 4.51 kbp 7.7 kbp 69 kbp 24 kbp
Scaffold N50 57.6 Mbp 384 Mbp 11.4 Mbp 2.0 Mbp
Total Assembly Size 4.23 Gbp 1.90 Gbp 4.09 Gbp 2.31 Gbp

NRGene Has Provided the IWGSC Global Wheat Consortium the Full Bread Wheat Reference Genome

Assembly Results of DeNovoMAGIC TM 3.0

Parameter DeNovoMAGIC™ 2.0 DeNovoMAGIC™ 3.0
Diploid Wheat
(Aegilops tauschii)		 Tetraploid Wheat
(Wild Emmer)		 Hexaploid Wheat
(Chinese Spring)		 Maize Strawberry
(Heterozygote Octoploid)		 Canola
Fold Coverage of
Short Reads
(PE&MP)		 200X 180X 180X 180X 190X 230X
Contig N50 5 10 14 2 2 2
Scaffold N50
(L50)		 11.4 Mbp
(106)		 7.0 Mbp
414)		 7.06 Mbp
(566)		 35.5 Mbp
(18)		 3.9 Mbp
(108)		 2.0 Mbp
(236)
Scaffold N90
(L90)		 2.27 Mbp
(405)		 1.15 Mbp
(1827)		 1.26 Mbp
(2363)		 11 Mbp
(58)		 37 Kbp
(1028)		 537 Kbp
(197)
Total Assembly Size 4.09 Gbp 10.50 Gbp 14.53 Gbp 2.13 Gbp 1.4 Gbp 1.04 Gbp
Unfilled Gaps(=n) 1.39% 1.63% 1.80% 1.90% 2.10% 0.94%